D-Luciferin sodium salt
The D-Luciferin sodium salt (order# BC218) is the sodium salt of the D-Luciferin free acid (order# S039)
D-Luciferin potassium salt
D-Luciferin potassium salt (order# BC219) is a highly water-soluble, premium-grade bioluminescent substrate extensively used as a reporter for the enzyme Firefly Luciferase (from Photinus pyralis). As a cornerstone of actual molecular biology and molecular imaging, it facilitates the sensitive detection of biological processes by converting chemical energy into light.
The mechanism and applications of D-Luciferin potassium salt (order# BC219) are typical in an ATP-dependent reaction, where luciferase catalyzes the oxidation of D-luciferin to oxyluciferin, yielding a high-intensity bioluminescent signal.
This reaction of the D-Luciferin potassium salt (order# BC219) is fundamental for:
- In Vivo Bioluminescence Imaging (BLI): Monitoring tumor growth, gene expression, and therapeutic efficacy in living animal models due to the substrate’s excellent bioavailability and tissue penetration.
- Reporter Gene Assays: Quantifying gene expression levels in transfected cell lines with high signal-to-noise ratios.
- ATP & Cell Viability Analysis: Detecting metabolic activity and infinitesimal levels of ATP in various biological samples.
- Pathogen Tracking: Real-time visualization of bacterial or viral infections in preclinical research.
Our D-Luciferin potassium salt BC219 (the potassium salt of the free acid S039) is wordwide used in the cancer research. A lot of researchers have used our high quality D-Luciferin potassium salt so far.
You can find more information by searching for the key words “materials and methods” together with “D-Luciferin” and “Synchem”.
The CoA BC219 is typical; high quality is guaranteed.
You can order and pay with creditcard using our shop or you may simple send us an email: [email protected]
The D-Luciferin free acid S039 and the sodium salt BC218 are also in stock and can be sent immediately.
Freight charge depends on the country and will be given during the order process.
Out of stock
Coelenterazine native
Coelenterazine is a luminophore found in aquatic organisms and is the substrate for many luciferase enzymes, including Renilla reniformis, Gaussia and aequorins. It is commonly used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.
D-Luciferin free acid
D-Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. The water soluble substrate for the firefly luciferase enzyme uses ATP and Mg-Ion as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death indicator.
Luciferin is a common reagent used throughout the biotechnology family and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. This is often made by the use of the D-Luciferin sodium salt (BC218), because of it`s good solubility in water and tolerance of living organism. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).
Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
Firefly Luciferin is identical to Beetle Luciferin.
References:
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.03097/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566035/
Out of stock
6-Amino-6-deoxyluciferin
biocompatible condensation reaction for labeling of terminal cysteine residues on proteins
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Coelenterazine hcp
L-Luciferin free acid
Out of stock
Coelenterazine h
BlueSyn C
Novel coelenterazine substrate for Oplophorus gracilirostris luciferase variant
A blue emitting Rluc substrate for BRET studies