Bis-ANS Dipotassium salt

4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic acid dipotassium salt (commonly known as Bis-ANS), is a powerful bifunctional fluorescent probe widely utilized in protein biochemistry. Characterized by its high sensitivity and photostability, this hydrophobic dye is essentially non-fluorescent in aqueous solutions but exhibits a dramatic increase in fluorescence intensity upon binding to nonpolar cavities or exposed hydrophobic regions of proteins.

The main applications are:

• Protein Folding & Conformational Studies: Bis-ANS is a standard tool for detecting intermediate folding states (such as “molten globules”) and monitoring structural perturbations.
• Aggregation Inhibition: Beyond its role as a probe, research suggests Bis-ANS possesses chaperone-like activity, effectively suppressing the aggregation of proteins like insulin and alcohol dehydrogenase by blocking solvent-exposed hydrophobic patches.
• Amyloid Research: It is used to characterize soluble Aβ conformations and tau oligomers, providing distinct fluorescent profiles that differentiate them from mature fibrils.
• Protein Quantification: Recent methodologies have utilized Bis-ANS for sensitive protein assays (range 0.28 to >100 μg/mL) that remain robust in the presence of detergents and inhibitors.

CoA BC046

MSDS BC046